Fig. 7
From: Optimizing production of Fc-amidated peptides by Chinese hamster ovary cells
Varying copper and ascorbate concentrations. a. Copper. Adding small amounts of CuSO4 did not increase amidation of GLP1 by endogenous CHO cell PAM or by exogenous PAM1, the amidation of NMU by exogenous PAM/820 s, or the amidation of PYY by exogenous PAM1. The base medium contained 5 nM Cu (Life Technologies website). b. Ascorbate. Adding a concentration of ascorbate equivalent to normal plasma levels (AscA: 50 μM) [56] did not increase amidation of GLP1, NMU or PYY by the same cell lines as in (a). The base medium did not contain ascorbic acid. For all three peptides, data from several assays were pooled