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Fig. 4 | BMC Biotechnology

Fig. 4

From: Optimizing production of Fc-amidated peptides by Chinese hamster ovary cells

Fig. 4

Subcellular localization of targeted PAM proteins. The indicated CHO cell lines were fixed, permeabilized and stained simultaneously with antisera to GFP (visualized using a FITC-tagged antibody) and to the indicated subcellular markers (visualized using a Cy3-tagged antibody). Cells expressing PAM/ER were stained for GFP and calnexin (a) or GFP alone (b). Cells expressing PAM/TGN were stained for GFP and giantin (c) or for GFP alone (d). Cells expressing PAM/Endo were stained for GFP and EEA1 (e) or for GFP alone (f). Epifluorescence images were taken using a 60X oil immersion lens

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