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Table 1 Optimization of the RNAi feeding regimen

From: Development of an efficient RNA interference method by feeding for the microcrustacean Daphnia

  1. We fed groups of 10 D. melanica in 100 ml of lake water on varying amounts of bacteria expressing the phenoloxidase dsRNA (P1) as indicated in the first column for a duration of 12 days. We recorded lethality (which is listed as the total number of Daphnia that had died from start of the experiment and overall % dead at the end of the experiment) as well as phenotypic response (which is listed as the total number of live Daphnia displaying a clear carapace on a particular day and overall % with clear carapace at the end of the experiment). The percentages were calculated based on starting number of individuals (10). Only the living Daphnia were scored for carapace color. The green rows and columns indicate the results representing the bacterial concentration that was selected for analysis of phenoloxidase mRNA levels on day 10. This was a concentration that exhibited the best phenotypic response