Fig. 3
From: Development of an efficient RNA interference method by feeding for the microcrustacean Daphnia
Daphnia fed on bacteria expressing the phenoloxidase dsRNA demonstrate a loss in melanin pigmentation. a and b Proportion of Daphnia demonstrating pigmentation loss phenotype after 10 days on an RNAi feeding regimen with BL21(DE3) and HT115(DE3) strain respectively. Following the feeding regimen, Daphnia were UV treated to induce melanin production and then observed under a dissecting scope for pigmentation phenotype. wt (melanica): n = 50, EV (L4440) n = 51, GFP (L44417): n = 50, P1 (P1/L4440): n = 55, P2 (P2/L4440): n = 56, P3 (P3/L4440): n = 57. These abbreviations denote which plasmid was transformed into the bacteria before administering as feed to Daphnia. The data represent five replicate experiments. P-values are as follows: * = 0.0005, ** = 0.0032, *** = 0.0002, # = 1.2 × 10−7, ## = 2.0 × 10−7, and ### = 0.00016. c Photographic representation of Daphnia from each group as indicated below each panel. The non-melanic species D. pulex is shown for comparison