Fig. 2
From: Development of an efficient RNA interference method by feeding for the microcrustacean Daphnia
dsRNA generated in E. coli can be detected in Daphnia. a Reverse transcriptase-PCR data. Lane 1: RNA isolated from Daphnia fed on bacteria containing L4440 without any insert (empty vector-EV), Lane 2: RNA isolated from Daphnia fed on bacteria containing GFP/L4440, Lane 3: GFP/L4440 plasmid DNA (template positive control), Lanes 4 and 5: reverse transcriptase negative control (the negative control without reverse transcriptase ensures lack of plasmid DNA contamination). GAPDH was used as an internal control for ascertaining that equal amounts of Daphnia mRNA were analyzed in lanes 1 and 2. b Quantitative real time PCR data. Real Time PCR was performed with the same samples as in panel A. P-value: * = 5 × 10−5