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Table 3 Germinality index of the 24 scFvs with affinities better than 10 nM

From: Isolation of nanomolar scFvs of non-human primate origin, cross-neutralizing botulinum neurotoxins A1 and A2 by targeting their heavy chain

 

Germinality Index (GI)

Clone name

Heavy chain

Light chain

Mean GI

A1HC58

84.44

91.01

87.72

A1HC81

82.41

92.13

87.27

A1HC65

78.01

95.5

86.75

A1HC33

82.41

91.01

86.71

A1HC62

83.51

89.77

86.64

A1HC32

83.33

89.53

86.43

A1HC26

80.21

92.13

86.17

A1HC45

80.21

92.13

86.17

A1HC3

79.12

92.85

85.98

A1HC67

85.71

86.04

85.87

A1HC17

80.21

89.88

85.04

A1HC74

84.61

85.21

84.91

A1HC38

82.22

86.51

84.36

A1HC64

78.02

89.88

83.95

A1HC68

78.02

89.88

83.95

A1HC49

80.21

87.64

83.92

A1HC31

78.02

89.77

83.89

A1HC34

79.12

88.37

83.74

A1HC43

83.51

83.52

83.52

A1HC7

78.02

88.76

83.39

A1HC39

79.12

86.51

82.81

A1HC80

76.92

86.36

81.64

A1HC6

90.69

71.91

81.3

A1HC47

79.12

83.14

81.13

  1. The Germinality Index (GI) of the 24 scFvs with the best affinities was calculated for each sequence (VH, VL, mean GI) using IMGT/DomainGapAlign tool. The scFvs were ranked according to their predictive tolerance. The scFvs in bold were of particular interest for this study