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Table 3 Germinality index of the 24 scFvs with affinities better than 10 nM

From: Isolation of nanomolar scFvs of non-human primate origin, cross-neutralizing botulinum neurotoxins A1 and A2 by targeting their heavy chain

  Germinality Index (GI)
Clone name Heavy chain Light chain Mean GI
A1HC58 84.44 91.01 87.72
A1HC81 82.41 92.13 87.27
A1HC65 78.01 95.5 86.75
A1HC33 82.41 91.01 86.71
A1HC62 83.51 89.77 86.64
A1HC32 83.33 89.53 86.43
A1HC26 80.21 92.13 86.17
A1HC45 80.21 92.13 86.17
A1HC3 79.12 92.85 85.98
A1HC67 85.71 86.04 85.87
A1HC17 80.21 89.88 85.04
A1HC74 84.61 85.21 84.91
A1HC38 82.22 86.51 84.36
A1HC64 78.02 89.88 83.95
A1HC68 78.02 89.88 83.95
A1HC49 80.21 87.64 83.92
A1HC31 78.02 89.77 83.89
A1HC34 79.12 88.37 83.74
A1HC43 83.51 83.52 83.52
A1HC7 78.02 88.76 83.39
A1HC39 79.12 86.51 82.81
A1HC80 76.92 86.36 81.64
A1HC6 90.69 71.91 81.3
A1HC47 79.12 83.14 81.13
  1. The Germinality Index (GI) of the 24 scFvs with the best affinities was calculated for each sequence (VH, VL, mean GI) using IMGT/DomainGapAlign tool. The scFvs were ranked according to their predictive tolerance. The scFvs in bold were of particular interest for this study