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Table 2 Summary of protein characteristics and post-translational modifications of pdFVII and rFVII from three cell lines

From: Differences in N-glycosylation of recombinant human coagulation factor VII derived from BHK, CHO, and HEK293 cells

  pdFVII CHOrFVII HEK293rFVII BHKrFVII
rFVII specific activity calculated from PT clotting values per mg protein (antigen) [1000 IU/mg] Not done, theoretical specific activity 2.0 kIU/mg 2.0 2.3 1.8
Peptide map Similar
γ-carboxylation of E35; i.e. abundance of 10th Gla [%] 82 % 53 % 56 % 53 %
Presence of propeptide Not detected by peptide mapping
Hydroxylation of D68 [%] <1 % 9 % 7 % 15 %
O-glycosylation on S52 / S60; only three major identified types shown [%] 31 %: Glc(Xyl)2 / Fuc 56 %: Glc(Xyl)2 / Fuc 62 %: Glc(Xyl)2 / Fuc 21 %: Glc(Xyl)2 / Fuc
27 %: GlcXyl / Fuc 7 %: GlcXyl2 7 %: GlcXyl / Fuc 50 %: Glc / Fuc
15 %: Glc / Fuc 14 %: Glc / Fuc 10 %: Glc / Fuc 12 %: Glc / -
  1. Peptide mapping and the PTMs were done by LC-MS after tryptic digest (see Methods). The raw data for γ-carboxylation and O-glycosylation of all FVII isolates can be found in the addional PDF file provided in Additional file 1: Tables S1 and S3, respectively. Legend: Fuc Fucose, Gla γ-carboxy glutamic acid, Glc Glucose, IU International units, Xyl Xylose