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Table 1 Productivities of rFVII from BHK-, CHO- and HEK293-clones after two and three cloning rounds

From: Differences in N-glycosylation of recombinant human coagulation factor VII derived from BHK, CHO, and HEK293 cells

Cell line Number of limited dilution cloning round Number of clones in first screening Number of clones tested for final characterization rFVII titer [IU/ml] rFVII cell-specific productivity [IU/106cells/day] Pre-activation: IU rFVIIa per IU rFVII [%]
BHK 2 96 6 0.7 ± 0.2 0.5 ± 0.1 2.5 ± 0.5
3 150 22 0.6 ± 0.3 1.1 ± 0.4 1.2 ± 0.9
CHO 2 143 7 1.0 ± 0.8 1.7 ± 1.8 8.3 ± 1.9
3 198 46 5.7 ± 3.5 6.7 ± 3.1 5.6 ± 2.6
HEK293 2 235 36 5.8 ± 3.0 4.7 ± 1,6 4.2 ± 2.9
3 132 35 6.7 ± 3.7 4.1 ± 2.4 11.6 ± 19.3
  1. Mean values and standard deviations are shown for finally characterized clones obtained by limited dilution cloning. rFVII secreted into cell-culture supernatants was determined using a PT clotting assay, and the amount of pre-activated rFVIIa by STA-clot FVIIa-rTF assay. Supernatants were harvested after 24-h secretion into medium not containing FBS (see Methods)