Cell line | Number of limited dilution cloning round | Number of clones in first screening | Number of clones tested for final characterization | rFVII titer [IU/ml] | rFVII cell-specific productivity [IU/106cells/day] | Pre-activation: IU rFVIIa per IU rFVII [%] |
---|---|---|---|---|---|---|
BHK | 2 | 96 | 6 | 0.7 ± 0.2 | 0.5 ± 0.1 | 2.5 ± 0.5 |
3 | 150 | 22 | 0.6 ± 0.3 | 1.1 ± 0.4 | 1.2 ± 0.9 | |
CHO | 2 | 143 | 7 | 1.0 ± 0.8 | 1.7 ± 1.8 | 8.3 ± 1.9 |
3 | 198 | 46 | 5.7 ± 3.5 | 6.7 ± 3.1 | 5.6 ± 2.6 | |
HEK293 | 2 | 235 | 36 | 5.8 ± 3.0 | 4.7 ± 1,6 | 4.2 ± 2.9 |
3 | 132 | 35 | 6.7 ± 3.7 | 4.1 ± 2.4 | 11.6 ± 19.3 |