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Fig. 3 | BMC Biotechnology

Fig. 3

From: Differences in N-glycosylation of recombinant human coagulation factor VII derived from BHK, CHO, and HEK293 cells

Fig. 3

a and b Mass spectra of the N-glycosylated peptides containing the light and heavy chain glycosylation sites (N145 and N322) of pdFVII and rFVII isolated from CHO, HEK293 and BHK. a Mass spectra of the light chain peptides (N145). Relative intensities of glycan structures were calculated based on the masses of the 3-fold positively charged peptides. b Mass spectra of the heavy chain peptides (N322) based on the 4-fold positively charged peptides. Both mass spectra of all samples were recorded within the same system setup. Only intensities of manually identified peaks were used for the calculation of relative amounts of N-glycan species. Major identified oligosaccharide structures are shown for each FVII isolate. Theoretical and calculated molecular masses, and relative abundances of glycan structures can also be found in the Additional file 1: Tables S4A-H

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