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Fig. 1 | BMC Biotechnology

Fig. 1

From: Differences in N-glycosylation of recombinant human coagulation factor VII derived from BHK, CHO, and HEK293 cells

Fig. 1

SDS-PAGE analysis of rFVII in cell culture supernatants and after purification. a Western blot after SDS-PAGE stained for human FVII: lane 1: molecular mass marker. pdFVII (lane 2), pdFVIIa (lane 3), commercially available rFVIIa (lane 4), supernatants from CHOrFVII clones (lanes 5–7) and from HEK293rFVII clones (lanes 8–10). 50 ng rFVII based on antigen were loaded per lane and resolved under reducing conditions by SDS-PAGE. Human FVII was detected by dual-antibody staining after blotting on a nitrocellulose membrane. Molecular mass marker sizes are shown on the left. b Silver-stained SDS-PAGE: 220 ng per lane each purified HEK293rFVII (lane 1), commercially available rFVIIa (lane 2), were resolved by SDS-PAGE and protein bands were made visible by silver staining. M: molecular mass standard, molecular mass marker sizes are shown on the left.. The FVII whole molecule, the heavy (HC) and light chain (LC) of rFVIIa are marked with arrows

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