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Fig. 2 | BMC Biotechnology

Fig. 2

From: Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris

Fig. 2

Expression of the phytase from C. amalonaticus CGMCC 1696 in P. pastoris. a: Time dependence of the activity of phytase, cell growth and phytase protein content of Gs115/Phy after induction with methanol, using GS115/HKA as the background (control) sample. b: SDS-PAGE assay of the culture supernatant containing C. amalonaticus CGMCC 1696 phytase (stained with Coomassie Blue) after methanol induction for 96 h. Lane 1: culture supernatants from recombinant P. pastoris GS115/HKA; lane 2: culture supernatants from recombinant P. pastoris Gs115/Phy; lane 3: culture supernatants from recombinant P. pastoris Gs115/Phy after PNGase F treatment; lane 4: culture supernatants from recombinant P. pastoris GS115/HKA after PNGase F treatment

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