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Fig. 3 | BMC Biotechnology

Fig. 3

From: Construction of polyomavirus-derived pseudotype virus-like particles displaying a functionally active neutralizing antibody against hepatitis B virus surface antigen

Fig. 3

Analysis of recombinant constructs VP1/scFv-Fc-VP2 (construct #1, lane 1) and VP1/scFv-VP2 (construct #2, lane 2) purified on CsCl gradient by SDS-PAGE (a) and Western blot with the MAb 3D10 (b) and anti-human IgG antibody (c). Protein band corresponding to MW of 78.4 kDa represents scFv-VP2 fused protein (lane 1), protein band of 52.5 kDa represents scFv-VP2 fused protein (lane 2) and protein band of 42 kDa represents VP1 protein (lanes 1–3). The construct VP1/GFP-VP2 (lane 3) and purified human IgG (lane 4) are used as positive controls for Western blot

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BMC Biotechnology

ISSN: 1472-6750

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