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Table 3 List of NP668/1 fermentation batches to investigate the optimal point of temperature shift

From: High-level expression of a recombinant active microbial transglutaminase in Escherichia coli

Batch #

OD600

LacZ1–8Met-MTGase (mg/l)

Wet mass (g)

LacZ1–8Met-MTGase (mg/g)

NP668/C001

58

1117

744

15,0

NP668/C002

64

2191

604

36,3

NP668/C003

56

3085

493

62,6

NP668/C004

51

1548

646

24,0

NP668/C005

58

2907

590

49,3

NP668/C006

60

2376

580

41,0

NP668/C007

58

2761

560

49,3

NP668/C008

42

2109

384

54,9

NP668/C009

54

2500

540

46,3

NP668/C010

53

2520

538

46,7

NP668/C013

60

2633

591

44,6

NP668/C014

59

2196

564

38,9

NP668/C015

60

1939

572

33,9

NP668/C016

50

2157

575

37,5

NP668/C017

37

1377

522

26,4

NP668/C018

33

2415

497

48,6

NP668/C019

32

1677

571

29,4

Mean

52

2206

563

40,3

Sd

10

541

74

12,0