Table 1 Fatty acid composition of yeast transformants containing the empty plasmid (pYES2) and recombinant plasmids (pPyDes6 and pMPyDes6) grown in SD medium supplemented with 50 μM fatty acid substrates
Yeast transformant | Fatty acid composition in total fatty acids (% w/w) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
C16:0 | C16:1∆9 | C16:2∆6,9 | C18:0 | C18:1∆9 | C18:2∆6,9 | C18:2∆9,12 (LA) | C18:3∆6,9,12 (GLA) | C18:3∆9,12,15 (ALA) | C18:4∆6,9,12,15 (STA) | |
pYES2 (control) | ||||||||||
No fatty acid addition | 22.1 ± 0.4 | 43.7 ± 0.5 | - | 7.5 ± 0.1 | 26.7 ± 0.2 | - | - | - | - | - |
+LA | 23.5 ± 0.3 | 34.3 ± 0.9 | - | 8.5 ± 0.1 | 20.2 ± 0.7 | - | 13.6 ± 1.2 | - | - | - |
+ALA | 22.7 ± 0.3 | 32.8 ± 0.3 | - | 8.7 ± 0.1 | 20.9 ± 0.6 | - | - | - | 14.9 ± 0.7 | - |
pPyDes6 | ||||||||||
No fatty acid addition | 19.1 ± 1.3 | 43.7 ± 3.6 | 1.4 ± 0.1 | 7.9 ± 0.5 | 27.8 ± 1.9 | - | - | - | - | - |
+LA | 22.4 ± 0.5 | 37.6 ± 0.4 | 0.2 ± 0.0 | 7.1 ± 0.2 | 21.1 ± 0.3 | - | 11.2 ± 0.0 | 0.6 ± 0.0 | - | - |
+ALA | 21.0 ± 2.1 | 29.9 ± 8.1 | 0.2 ± 0.1 | 5.8 ± 2.0 | 28.9 ± 11.4 | - | - | - | 13.8 ± 1.0 | 0.6 ± 0.2 |
pMPyDes6 | ||||||||||
No fatty acid addition | 22.6 ± 0.2 | 31.9 ± 0.1 | 11.4 ± 0.2 | 7.7 ± 0.0 | 23.3 ± 0.1 | 3.1 ± 0.0 | - | - | - | - |
+LA | 24.8 ± 0.2 | 26.6 ± 0.0 | 5.7 ± 0.3 | 9.6 ± 0.1 | 20.8 ± 0.0 | 1.6 ± 0.0 | 4.1 ± 0.1 | 6.9 ± 0.1 | - | - |
+ALA | 24.6 ± 0.3 | 25.2 ± 0.2 | 5.9 ± 0.0 | 10.3 ± 0.1 | 20.3 ± 0.4 | 1.6 ± 0.1 | - | - | 4.7 ± 0.3 | 7.3 ± 0.1 |