Skip to main content

Table 3 Substrate specificity for oligosaccharides of α-glucosidase TtAG, AglHHB27, AglHHB8 and TcaAG from Thermus strains

From: Evaluation and directed evolution for thermostability improvement of a GH 13 thermostable α-glucosidase from Thermus thermophilus TC11

Substrate Glycosidic linkage Relative activity (%)
TtAG AglHHB27 AglHHB8 TcaAG
Sucrose Glu-α-1,2-Fru 47.8 (46.1) 12.5 (8.0) 8.4 59.3
Trehalose Glu-α-1,1-Glu 103.7 (100.0) 156.7 (100.0) 0.0 0.0
Isomaltose Glu-α-1,6-Glu 100.0 (96.5) 100.0 (63.8) 100.0 100.0
Cellobiose Glu-β-1,4-Glu 0.0 0.0 0.0 0.0
Melibiose Gla-α-1,6-Glu 0.0 0.0 0.0 0.0
Maltose Glu-α-1,4-Glu 3.7 (3.5) 15.1 (9.7) 2.8 1.6
Melizitose Glu-[α-1,3]-Fru-β-2,1-Glu 0.0 0.0 0.0 0.0
Isomaltotriose Glu-[α-1,6]-Glu-α-1,6-Glu 188.2 (181.6) 47.8 (30.5) 45.2 4.8
Raffinose Gla-[α-1,6]-Glu-α-1,2-Glu 0.0 0.0 0.0 0.0
Panose Glu-[α-1,6]-Glu-α-1,4-Glu 62.5 (60.3) 56.1 (35.8) 42.1 44.6