Preparation | Total protein (mg) | Total activity (U) | Yield (%) | Specific activity (U/mg) | Purification fold |
---|---|---|---|---|---|
Supernatant of crude extract | 89.4 | 2772.2 | 100.0 | 31.01 | 1.0 |
Supernatant after heating treatment | 25.5 | 3312.0 | 119.0 | 129.88 | 4.2 |
Affinity (His-Tag column) | 8.3 | 2398.4 | 86.5 | 288.96 | 9.3 |