Skip to main content

Table 1 Purification of recombinant TtAG

From: Evaluation and directed evolution for thermostability improvement of a GH 13 thermostable α-glucosidase from Thermus thermophilus TC11

Preparation

Total protein (mg)

Total activity (U)

Yield (%)

Specific activity (U/mg)

Purification fold

Supernatant of crude extract

89.4

2772.2

100.0

31.01

1.0

Supernatant after heating treatment

25.5

3312.0

119.0

129.88

4.2

Affinity (His-Tag column)

8.3

2398.4

86.5

288.96

9.3