Skip to main content
Fig. 2 | BMC Biotechnology

Fig. 2

From: Evaluation and directed evolution for thermostability improvement of a GH 13 thermostable α-glucosidase from Thermus thermophilus TC11

Fig. 2

TLC and HPLC chromatogram analysis of transglycosylation products. a TLC analysis of transglycosylation products when sucrose and trehalose at 300 mM were used as substrates for different incubation time. Lane 1, sucrose for 3 h; lane 2, sucrose for 10 h; lane 3, sucrose for 20 h; lane 4, trehalose for 3 h; lane 5, trehalose for 10 h; lane 6, trehalose for 20 h; lane M, marker. G1, glucose; G2, maltose; G3, maltotriose; G4, maltotetraose; G5, maltopentaose. b and (c) HPLC chromatogram of the reaction mixture after 20 h of incubation when sucrose or trehalose was used as the substrate. F, fructose; G, glucose; S, sucrose; T, trehalose. The arrows indicate the new transglycosylation products

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us