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Fig. 3 | BMC Biotechnology

Fig. 3

From: Immobilization of trypsin in organic and aqueous media for enzymatic peptide synthesis and hydrolysis reactions

Fig. 3

Reusability of trypsin immobilized onto M-PVA in organic solvent in synthesis reaction (♦) and hydrolysis reaction (▲). One cycle of synthesis reaction corresponds to one hour of peptide synthesis reaction in 80 % ethanol at 20 °C, one cycle of hydrolysis reaction corresponds to 30 min in Tris buffered solution pH 8 at 30 °C. Linear trend lines show possible end values of activity for synthesis (—) and hydrolysis (− − −) reactions

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BMC Biotechnology

ISSN: 1472-6750

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