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Table 1 Polymerase chain reaction primers used in this study

From: An avian influenza A (H7N9) virus vaccine candidate based on the fusion protein of hemagglutinin globular head and Salmonella typhimurium flagellin

Primers Sequence Restricted site
HA1-2-F1 5′-CCCGAGCTCAAAGGGAAAAGGACAGTTGACC-3′ SacI
HA1-2-R1 5′-CCCAAGCTTGGCATCAACCTGTACT-3′ Hind III
HA1-2-F2 5′-CCGGAATTCAAAGGGAAAAGGACAGTTGACC-3′ EcoRI
HA1-2-R2 5′-CACCTCCGCTTCCACCTCCACCGGCATCAACCTGTACT-3′ (Gly4Ser)3
fliC-F 5′-AGGTGGAAGCGGAGGTGGTGGAAGCATGGCACAAGT CATTAATA-3′ (Gly4Ser)3
fliC-R 5′-CCGTCTAGATTAACGCAGTAAAGAGAGGACG-3′ XbaI