Fig. 4
From: Hybrid flagellin as a T cell independent vaccine scaffold
Hybrid flagellin filaments are able to crosslink BCR in vitro. a Schematic of how TI immune response is generated. Polymeric antigens bind to B cell receptors and crosslink them. Once this crosslinking event occurs, the B cell will self-differentiate into plasma cells to produce antibodies. b 4G2 B cell hybridoma (DENV2-specific IgG producing hybridoma) stimulated with polymeric hFliC. Stimulated cells showed more organized expression of Ig receptors compared to unstimulated cells, indicating cells were crosslinked (top panel). This was further confirmed by staining with anti-Histag antibody