Table 3 Primers used in this study
Primer | Sequence (5′-3′) |
|---|---|
1296P1 | CCCAAGCTTGCCAGCGGCGGCGACG (underlined bases correspond to Hind III site) |
1296P2 | CGGGGTACCTTACGACCAGCCCTGCTTCACCTC (underlined bases correspond to Kpn I site) |
1297P1 | TTGGGCCGTTCTGGCCGCCAGCGGCGGCGACG (underlined bases correspond to Sfi I site) |
1297P2 | CGCGGATCCTTACGACCAGCCCTGCTTCACCTC (underlined bases correspond to BamH I site) |
7N160Q1 | CCCCAGGAGACGCAAGCCGAGTTT (underlined bases encode Gln) |
7N160Q2 | GCGTGGCCTGCTGTTTTCCAGGTC |
7N355Q1 | CGGCAGTGGTCTGCCGGGTA (underlined bases encode Gln) |
7N355Q2 | GAACTTGCTCTCGTATACGTTCATGGG |