Overproduction of pro-transglutaminase from Streptomyces hygroscopicus in Yarrowia lipolytica and its biochemical characterization

Table 2 Extracellular expression of Streptomyces TGases in heterologous systems

Reference	Gene resource	Host strain	Antibiotic resistance for selection	Yield (U/mL)	Productivity (U/mL/h)
Yurimoto et al. [9]	Streptoverticillium mobaraense S-8112	C. boidinii BUL	Amp^R	1.8	0.02
Lin et al. [32]	S. platensis M5218	S. lividans JT46	Tsr^R	2.2	0.03
Liu et al. [33]	S. fradiae	S. fradiae	Apr^R	3.2	0.07
Date et al. [8]	Sv. mobaraense	C. glutamicum YDK010	Kan^R	16.3	0.41
Noda et al. [34]	Sv. cinnamoneum	S. lividans 1326	Amp^R/Tsr^R	2.8	0.02
Liu et al. [27]	S. hygroscopicus	E. coli BL21(DE3)	Amp^R	4.5	0.11
This work	S. hygroscopicus	Y. lipolytica Po1h	none (selection by Ura⁻ auxotrophy complementation)	35.3	0.30

Abbreviation: Amp^R, ampicillin resistance; Tsr^R, thiostrepton resistance; Apr^R, apramycin resistance; Kan^R, kanamycin resistance; Ura⁻, uracil auxotrophy

ISSN: 1472-6750