Fig. 5From: Overproduction of pro-transglutaminase from Streptomyces hygroscopicus in Yarrowia lipolytica and its biochemical characterizationSDS-PAGE analysis of purified pro-TGase and its variants. Culture supernatants containing pro-TGase or its variants were obtained by centrifugation and purified by ion exchange chromatography on a Fractogel EMD SO3− (see “Methods”). M: protein marker, 1: wild-type pro-TGase (pINA1297/pro-TGase), 2: N160Q, 3: N355Q, 4: N160Q/N355QBack to article page