Fig. 2

Pro-TGase production by yeast transformants. a SDS-PAGE analysis of pro-TGase secretion by recombinant Y. lipolytica strains carrying the following constructs. b Extracellular yield of pro-TGase and its variants produced by the corresponding recombinant Y. lipolytica strains. Labeling for both (a) and (b): 1: pINA1296, 2: wild-type pro-TGase (pINA1296/pro-TGase), 3: pINA1297, 4: wild-type pro-TGase (pINA1297/pro-TGase), 5: N160Q, 6: N355Q, 7: N160Q/N355Q, M: protein marker. All of the transformants were cultivated at 28 °C and 200 rpm for 5 days using a modified PPB medium; the TGase activities of pro-TGase and its variants were obtained following in vitro activation (see “Methods”). Each value represents the mean of three independent measurements