Fig. 3

Phylogenetic tree of the four Leptosphaerulina sp. laccase-type proteins and selected fungal laccases, ferroxidases, ascorbate oxidases, and fungal pigment MCOs. The optimal tree constructed by the Neighbor-Joining method based on MUSCLE alignment (L2-L3 sequences length) is shown. Percentages over 50 % of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. The evolutionary distances were calculated by the JTT matrix-based method. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree (number of amino acid substitutions per site). All positions containing gaps and missing data were eliminated. Evolutionary analyses were conducted in MEGA6. The GenBank accession numbers for the protein sequences are depicted together with the acronyms for the fungal strains. AAJ: Auricularia auricula-judae; ABE: Arthroderma benhamia; ACA: Ajellomyces capsulatus; ADE: Ajellomyces dermatitidis; AFL: Aspergillus flavus; AFU: Aspergillus fumigatus; AGY: Arthroderma gypseum; AKA: Aspergillus kawachii; ANI: Aspergillus nidulans; AOR: Aspergillus oryzae; APO: Auricularia polytricha; CAQ: Clavariopsis aquatica; CCI: Coprinopsis cinerea; CGA: Coriolopsis gallica; CGL: Chaetomium globosum; CGR: Colletotrichum graminicola; CLU: Curvularia lunata; DSQ: Dichomitus squalens; EDE: Exophiala dermatitidis; FFU: Fusarium fujikuroi; FOX: Fusarium oxysporum; LMA: Lepthospaheria maculans; MAL: Melanocarpus albomyces; MOS: Mixia osmundae; MTH: Myceliophtora thermophila; NCR: Neurospora crassa; PCH: Phanerochaete chrysosporium; PER: Pleurotus eryngii; PFL: Pleurotus florida; PNO: Phaeosphaeria nodorum; PSP: Phaeosphaeria spartinicola; PTE: Pyrenophora teres; PTR: Pyrenophora tritici-repentis; SAE: Stropharia aeruginosa; SCO: Schizophyllum commune; STA: Stagonospora sp.; STU: Setosphaeria turcica; TAR: Theilavia arenaria; TEQ: Trichophyton equinum; TCI: Trametes cinnabarina; TVI: Trametes villosa; POS: Pleurotus ostreatus; TRE: Trichoderma reesei; TTR: Trametes trogii; LBI: Lacaria bicolor; PHA: Phaeosphaeria halima; PPL: Postia placenta; VAL: Verticillium alfalfae. The tree was rooted by two insect laccase sequences from Manduca sexta (MSE) and Tribolium castaneum (TCA)