Fig. 2

Partial protein sequences encoded by the four laccase-like genes, lac1, lac2, lac3 and lac4 amplified from Leptosphaerulina sp genomic DNA. The laccase signatures containing the copper ligands (magenta) are indicated; the peptide disclosed by proteomic analysis of the purified laccase is underlined in black, and the sequence for the corresponding degenerate peptide-based primer is highlighted in grey. Cys residues involved in one conserved S-S bridge in ascomycete laccases are highlighted in yellow