Table 1 Oligonucleotide primers used for the real-time PCR assays, the DNA walking approaches and the PCR confirmation of the transgenic junctions
From: Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices
Methods | Oligonucleotide names | Oligonucleotide sequences | Product sizes (bp) | References |
|---|---|---|---|---|
SYBR®Green qPCR | p35S F | AAAGCAAGTGGATTGATGTGATA | 75 | [6] |
| Â | p35S R | GGGTCTTGCGAAGGATAGTG | [6] | |
SYBR®Green qPCR | tNOS F | GATTAGAGTCCCGCAATTATACATTTAA | 69 | [6] |
| Â | tNOS R | TTATCCTAGKTTGCGCGCTATATTT | [6] | |
SYBR®Green qPCR | t35S pCAMBIA c-F | CGGGGGATCTGGATTTTAGTA | 137 | [14] |
| Â | t35S pCAMBIA a-R | AGGGTTCCTATAGGGTTTCGCTC | [14] | |
DNA Walking | p35S-F a (p35S R) | GGGTCTTGCGAAGGATAGTG | Â | [6] |
| Â | p35S-F b | TGTGCGTCATCCCTTACGTCAGT | / | This study |
| Â | p35S-F c | TATCACATCAATCCACTTGCTTT | Â | [6] |
DNA Walking | p35S-R a (p35S F) | AAAGCAAGTGGATTGATGTGATA | Â | [6] |
| Â | p35S-R b | ACTGACGTAAGGGATGACGCACA | / | This study |
| Â | p35S-R c | CACTATCCTTCGCAAGACCC | Â | [6] |
DNA Walking | tNOS-F a (tNOS F) | GATTAGAGTCCCGCAATTATACATTTAA | Â | [6] |
| Â | tNOS-F b | TTAATACGCGATAGAAAACAAAAT | / | This study |
| Â | tNOS-F c | AAATATAGCGCGCAAMCTAGGATAA | Â | [6] |
DNA Walking | tNOS-R a (tNOS R) | TTATCCTAGKTTGCGCGCTATATTT | Â | [6] |
| Â | tNOS-R b | ATTTTGTTTTCTATCGCGTATTAA | / | This study |
| Â | tNOS-R c | TTAAATGTATAATTGCGGGACTCTAATC | Â | [6] |
PCR junction | Rice chromosome II | CCCCTAATTTCTCACAGGCC | 848 | This study |
| Â | tNOS-F c | AAATATAGCGCGCAAMCTAGGATAA | [6] | |
PCR junction | Rice chromosome III | AGGTACTCAAGCCTTTTCCAGC | 1105 | This study |
| Â | tNOS-F c | AAATATAGCGCGCAAMCTAGGATAA | [6] |