Fig. 4From: Prokaryotic expression and mechanism of action of α-helical antimicrobial peptide A20L using fusion tagsMembrane permeabilization assay of the antimicrobial peptide A20L. (a) outer membrane permeabilization induced by A20L was detected by NPN uptake in P. aeruginosa ATCC 27853; (b) the effect of A20L on the inner membrane of E. coli ML-35. Release of cytoplasmic β-galactosidase activity (measured from the OD420 nm) from E. coli ML-35 treated with A20LBack to article page