Fig. 4

Membrane permeabilization assay of the antimicrobial peptide A20L. (a) outer membrane permeabilization induced by A20L was detected by NPN uptake in P. aeruginosa ATCC 27853; (b) the effect of A20L on the inner membrane of E. coli ML-35. Release of cytoplasmic β-galactosidase activity (measured from the OD_{420 nm}) from E. coli ML-35 treated with A20L