Fig. 1

The cloning and cleavage strategy for Ub-A20L and SUMO1/2/3/4-A20L. (a) pHUE-A20L, which was constructed by inserting the A20L gene at the BamHI/EcoRI sites of pHUE; (b) pET28b + −SUMO1/2/3/4-A20L, which was constructed by inserting the A20L gene at the BamHI/EcoRI sites of pET-28b+; (c) the cleavage strategy for Ub-A20L and SUMO-A20L