Fig. 1
From: Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast
Analysis of the production of novel HPyV-derived VP1 proteins in yeast using Coomassie blue-stained SDS-PAGE. In lanes: 1- in all gels, negative control sample of whole crude lysate of yeast transformed with pFX7 plasmid; 2 - whole lysate of yeast transformed with pFX7-KIPyV-VP1 (a), pFX7-WUPyV-VP1 (b), pFX7-MCPyV-VP1 (c), pFX7-HPyV6-VP1 (d), pFX7-HPyV7-VP1 (e), pFX7-TSPyV-VP1 (f), pFX7-HPyV9-VP1 (g), pFX7-HPyV10-VP1 (h), pFX7-STLPyV-VP1 (i), or pFX7-NJPyV-VP1 (j) plasmid; 3 - the soluble fraction recovered after centrifugation of whole lysate of yeast transformed with pFX7-KIPyV-VP1 (a), pFX7-WUPyV-VP1 (b), pFX7-MCPyV-VP1 (c), pFX7-HPyV6-VP1 (d), pFX7-HPyV7-VP1 (e), pFX7-TSPyV-VP1 (f), pFX7-HPyV9-VP1 (g), pFX7-HPyV10-VP1 (h), pFX7-STLPyV-VP1 (i), or pFX7-NJPyV-VP1 (j) plasmid; 4 - purified KIPyV VP1 (a), WUPyV VP1 (b), MCPyV VP1 (c), HPyV6 VP1 (d), HPyV7 VP1 (e), TSPyV VP1 (f), HPyV9 VP1 (g), HPyV10 VP1 (h), STLPyV VP1 (i), or NJPyV VP1 (j) protein. In all gels, pre-stained protein weight marker (Thermo Fisher Scientific Baltics) was loaded into lane M