Table 3 Semi-quantitative analysis of fusion construct expression and solubility in Rosetta2(DE3)pLysSa
From: Cold shock induction of recombinant Arctic environmental genes
| Â | Target size (kDa) | Mean Expression | Mean Solubility | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Candidate | Native | Recombinant | His | His-SUMO | His-TRX | His-MBP | His-TF | His | His-SUMO | His-TRX | His-MBP | His-TF |
MZ0003 | 48 | 45.6 | 3 | 3 | 0.5 | 3 | 3 | 1 | 0.5 | 1 | 1.5 | 3 |
MZ0009 | 48 | 35.8 | 3 | 3 | −1 | 3 | 3 | 1 | 1 | 0 | 2 | 3 |
MZ0012 | 42 | 41.6 | 3 | 3 | 2.5 | 3 | 3 | 0.5 | 0.5 | 0.5 | 1.5 | 3 |
MZ0013 | 39 | 21 | 3 | 3 | 2.5 | 3 | 3 | 1 | 1.5 | 1 | 3 | 3 |
MZ0047 | 32 | 28.9 | 2 | 2.5 | 1.5 | 2.5 | 3 | 1 | 0.5 | 1 | 2 | 3 |