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Table 3 Semi-quantitative analysis of fusion construct expression and solubility in Rosetta2(DE3)pLysSa

From: Cold shock induction of recombinant Arctic environmental genes

  Target size (kDa) Mean Expression Mean Solubility
Candidate Native Recombinant His His-SUMO His-TRX His-MBP His-TF His His-SUMO His-TRX His-MBP His-TF
MZ0003 48 45.6 3 3 0.5 3 3 1 0.5 1 1.5 3
MZ0009 48 35.8 3 3 −1 3 3 1 1 0 2 3
MZ0012 42 41.6 3 3 2.5 3 3 0.5 0.5 0.5 1.5 3
MZ0013 39 21 3 3 2.5 3 3 1 1.5 1 3 3
MZ0047 32 28.9 2 2.5 1.5 2.5 3 1 0.5 1 2 3
  1. aevaluated in two independent experiments by a qualitative scoring system described in the text