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Table 1 Summary of in silico analysis and cloning information of candidate sequences

From: Cold shock induction of recombinant Arctic environmental genes

Candidate Putative activity tBlast homologue (% identity)a PDB homologue (residue range in aa, % identity)b Rare codons (%)c SignalP prediction result for Gram negative/Gram positive (cleavage position) Molecular mass native/recombinant (Kda) European nucleotide archive accession number
MZ0003 Carbohydrate esterase Family 15 Acetyl xylan esterase Planctomyces maris (57) 3picB (39–430, 20.8) 6 N/Y(25–26) 48/46 LM651246
MZ0009 Glycosyl hydrolases family 18 Glycoside hydrolase Herpetosiphon aurantiacus DSM 785 (26) 3g6mA (27–358, 23.0) 12 N/Y(28–29) 48/36 LM651247
MZ0012 Glycosyl hydrolases family 39 β-xylosidase Mycobacterium abscessus (42) 4ekjA (65–358, 26.3) 13 N/N 42/42 LM651248
MZ0013 Carbohydrate esterase Family 4 Polysaccharide deacetylase Halorhodospira halophila SL1 (64) 3vus2A (31–259, 26.4) 6 Y/Y(30–31) 39/21 LM651249
MZ0047 ATP dependent DNA ligase ATP dependent DNA ligase Marinobacter adhaerens HP15 (70) 4d05A (39–287, 46.6) 8 Y(33–34)/Y(31–32) 32/29 LM651250
  1. aBased on Pfam and NCBI conserved domain annotations and BLAST homology
  2. bDetermined from SwissModel structure homology
  3. cDetermined from the Codon Frequency Distribution analysis tool (GenScript) and includes codons with values lower than 30 that are likely to hamper the expression efficiency