Table 1 Summary of in silico analysis and cloning information of candidate sequences
From: Cold shock induction of recombinant Arctic environmental genes
Candidate | Putative activity | tBlast homologue (% identity)a | PDB homologue (residue range in aa, % identity)b | Rare codons (%)c | SignalP prediction result for Gram negative/Gram positive (cleavage position) | Molecular mass native/recombinant (Kda) | European nucleotide archive accession number |
|---|---|---|---|---|---|---|---|
MZ0003 | Carbohydrate esterase Family 15 | Acetyl xylan esterase Planctomyces maris (57) | 3picB (39–430, 20.8) | 6 | N/Y(25–26) | 48/46 | LM651246 |
MZ0009 | Glycosyl hydrolases family 18 | Glycoside hydrolase Herpetosiphon aurantiacus DSM 785 (26) | 3g6mA (27–358, 23.0) | 12 | N/Y(28–29) | 48/36 | LM651247 |
MZ0012 | Glycosyl hydrolases family 39 | β-xylosidase Mycobacterium abscessus (42) | 4ekjA (65–358, 26.3) | 13 | N/N | 42/42 | LM651248 |
MZ0013 | Carbohydrate esterase Family 4 | Polysaccharide deacetylase Halorhodospira halophila SL1 (64) | 3vus2A (31–259, 26.4) | 6 | Y/Y(30–31) | 39/21 | LM651249 |
MZ0047 | ATP dependent DNA ligase | ATP dependent DNA ligase Marinobacter adhaerens HP15 (70) | 4d05A (39–287, 46.6) | 8 | Y(33–34)/Y(31–32) | 32/29 | LM651250 |