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Fig. 5 | BMC Biotechnology

Fig. 5

From: High-level expression of human arginase I in Pichia pastoris and its immobilization on chitosan to produce L-ornithine

Fig. 5

Conversion conditions of the immobilized ARG I reaction. a Relative activity of the arginase catalytic reaction following fixation by chitosan cross-linking with different concentrations of glutaraldehyde (ranging from 0.5 to 2.5 %), taking the activity of the concentration of 1 % as 100 %; b The influence of the concentration of Mn2+ in the reaction on the relative transformation efficiency; c The influence of reaction time on the conversion rate; d Operational stability of immobilized ARG I. All of the reactions were performed at 40 °C and pH10

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