Fig. 5
The variation of tone and TLC analysis with different feeding media in the fed-batch culture. a The rate of intracellular yellow pigments to red pigments (Y/R). b The rate of intracellular yellow pigments to orange pigments (Y/O). c TLC atlas in visible light. Lane 1-4: intracellular samples with four different feeding media (from No.1 to No.4) in the 5th days; Lane 5-8: intracellular samples with four different feeding media (from No.1 to No.4) in the 16th days; a and b presented the two yellow pigments at R f values of 0.75 and 0.71, c and d presented the two orange pigments at R f values of 0.67 and 0.63. d The scanning spectrum of the novel yellow substance