Table 2 Primer and adapter sequences used in this study
From: Feasibility of Cowpea chlorotic mottle virus-like particles as scaffold for epitope presentations
Construct | Primer set 5ˊ → 3ˊ |
|---|---|
CCMV-CP | GCGCCATGGGTACAGTCGGAACAGGGAAGTTAACTC (F) |
GGAAGCTTCACTAATACACCGGAGTG (R) | |
NΔ24CP | GGGCCATGGGGACTCGTGTGGTCCAAC (F) |
GGAAGCTTCACTAATACACCGGAGTG (R) | |
His-LBC | CGCACTAGTCATCACCATCACCATCACGAGCTCGCTGCCGAAGCTAAAGTAACCTCG (F) |
CGCACTAGTCGCACAAGAGGCGGTCCAC (R) | |
His-LDE | CGCACTAGTCATCACCATCACCATCACGAGCTCCCCAGTGTTAGTGGCACAGTGAAATC (F) |
CGCACTAGTAAGCAACCCAAGCCATAATAAAACTCTACC (R) | |
His-LFG | CGCACTAGTCATCACCATCACCATCACGAGCTCTCGAAAGATGTTGTCGCTGCTATGTAC (F) |
CGCACTAGTGTTGTCGGCCACAGCTAATGCC (R) | |
His-LHI | CGCACTAGTCATCACCATCACCATCACGAGCTCGCGGCTCTCACTGAGGGCGAC (F) |
CGCACTAGTACTGCTGTACAAGTAGATCGTTAAATCCGC (R) | |
M2e (7) loop adaptor | CTAGTCTGCTGACCGAAGTGGAGCT (F) |
CCACTTCGGTCAGCAGA (R) | |
M2e (15) loop adaptor | CTAGTCTGCTGACCGAAGTGGAAACCCCGACCCGCAACGGCTGGGAGCT (F) |
CCCAGCCGTTGCGGGTCGGGGTTTCCACTTCGGTCAGCAGA (R) | |
M2e (23) loop adaptor | CTAGTAGCCTGCTGACCGAAGTGGAAACCCCGACCCGCAACGGCTGGGAATGCCGCTGCAGCGAT |
AGCAGCGATGAGCT (F) | |
CATCGCTGCTATCGCTGCAGCGGCATTCCCAGCCGTTGCGGGTCGGGGTTTCCACTTCGGTCAGCA | |
GGCTA (R) | |
His-CP* | GGGCCATGGGTCACCACCACCACCACCACACAGTCGGAACAGGGAAGTTAAC (F) |
CCCAAGCTTCACTAATACACCGGAGTGAAAGAGTCGTCAAACGTAGGTCTGAC (R) | |
CP-His* | CGCCCATGGGTACAGTCGGAACAGGGAAGTTAACTCGTGCACAACGAAGGGC (F) |
GGGAAGCTTCACTAGTGGTGGTGGTGGTGGTGATACACCGGAGTGAAAGAGTCGTC (R) | |
His-CP-His* | GGGCCATGGGTCACCACCACCACCACCACACAGTCGGAACAGGGAAGTTAAC (F) |
GGGAAGCTTCACTAGTGGTGGTGGTGGTGGTGATACACCGGAGTGAAAGAGTCGTC (R) | |
His- NΔ24CP * | GGGCCATGGGTCACCACCACCACCACCACACTCGTGTGGTCCAACC (F) |
CCCAAGCTTCACTAATACACCGGAGTGAAAGAGTCGTCAAACGTAGGTCTGAC (R) | |
HA- NΔ24CP* | GGGCCATGGGGTGGACCATTCTGAAACCGAACGATGCGATTAACTTTGAAAGCAACCCCGGGACTCGTGTGGTCCAACCTG (F) |
CCCAAGCTTCACTAATACACCGGAGTGAAAGAGTCGTCAAACGTAGGTCTGAC (R) | |
His-HA- NΔ24CP* | GGGCCATGGGGCACCACCACCACCACCACTGGACCATTCTGAAACCGAAC (F) |
CCCAAGCTTCACTAATACACCGGAGTGAAAGAGTCGTCAAACGTAGGTCTGAC (R) | |
M2e(23) NΔ24CP* | CAACGGCTGGGAATGCCGCTGCAGCGATAGCAGCGATCCCGGGACTCGTGTGGTCCAACCTG (F1) |
GGGCCATGGTCATGGTCAGCCTGCTGACCGAAGTGGAAACCCCGACCCGCAACGGCTGGGAATGCCGCTGC (F2) | |
CCCAAGCTTCACTAATACACCGGAGTGAAAGAGTCGTCAAACGTAGGTCTGAC (R) | |
CP-HA* | CGCCCATGGGTACAGTCGGAACAGGGAAGTTAACTCGTGCACAACGAAGGGC (F) |
CGTTCGGTTTCAGAATGGTCCAATACACCGGAGTGAAAGAGTCG (R1) | |
CCCAAGCTTCACTAGTTGCTTTCAAAGTTAATCGCATCGTTCGGTTTCAGAATGG (R2) | |
CP-M2e(23)* | CGCCCATGGGTACAGTCGGAACAGGGAAGTTAACTCGTGCACAACGAAGGGC (F) |
CCCAGCCGTTGCGGGTCGGGGTTTCCACTTCGGTCAGCAGGCTATACACCGGAGTGAAAGAG (R1) | |
CCCAAGCTTCACTAATCGCTGCTATCGCTGCAGCGGCATTCCCAGCCGTTGCGGGTCGGG (R2) | |
VP1-CP/ VP1 NΔ24CP Adaptor | CATGGTACGGTGACACCAGCACTAACAACGTGAGAGGTGACCTTCAAGTGTTAGCTCAGAAGGCAGAAAGAACTCTGCCTGGGTCGA (F) |
CTAGTCGACCCAGGCAGAGTTCTTTCTGCCCTCTGAGCTAACACTTGAAGGTCACCTCTCACGTTGTTAGTGCTGGTGTCACCGTAC (R) | |
2C-CP/2C NΔ24CP adaptor | CATGGGAATGGCTGGACAACGCGCGTCAAGCGGGGTCGACGCGTGGGA (F) CTAGTCCCACGCGTCGACCCCGCTTGACGCGCGTTGTCCAGCCATTCC (R) |
26GNVΔ24CP* | GATACCGGCGAAGTGATTCTGAACAGCTATCGCATTAACCATTATCGCACTCGTGTGGTCCAACC (F1) |
CGCCCATGGGGAGAGTTGAATGCGAAATTGCGCTGAACAAAGATACCGGCGAAGTG (F2) | |
CCCAAGCTTCACTAATACACCGGAGTGAAAGAGTCGTCAAACGTAGGTCTGAC (R) | |
31GCVΔ24CP* | GAAGAATGGGGCTGCCTGGCGATTAACGATGGCTGCCTGTATGGCAGCTGCCAGGATATTACTCGTGTGGTCCAACC (F1) |
CGCCCATGGGGTTTAGCAAAGAACGCAGCAGCAACTGGGGCTGCGAAGAATGGGGCTGC (F2) | |
CCCAAGCTTCACTAATACACCGGAGTGAAAGAGTCGTCAAACGTAGGTCTGAC (R) | |
Δ24CP15GN* | GGGCCATGGGGACTCGTGTGGTCCAAC (F) |
CTTTCACGCAGCATTCGCTCACATACACCGGAGTG (R1) | |
GGGAAGCTTCTAGCTTTTAATAATGCTAATATCATCTTTCACGCAGC (R2) | |
Δ24CP16GC* | GGGCCATGGGGACTCGTGTGGTCCAAC (F) |
CATTCTTCGCAGCCCCAGTTGCTGCTATACACCGGAGTG (R1) | |
GGGAAGCTTCTAATCGTTAATCGCCAGGCAGCCCCATTCTTCGCAG (R2) |