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Fig. 6 | BMC Biotechnology

Fig. 6

From: Feasibility of Cowpea chlorotic mottle virus-like particles as scaffold for epitope presentations

Fig. 6

Expression of loop-inserted and terminal fusion CCMV constructs in E. coli. (a) SDS-PAGE analysis of cell extract (CE) and reassembled VLP pellet (RP) from the M2e(23) loop-inserted CCMV-CP (A1) βB-βC-M2e(23), (A2) βD-βE-M2e(23), (A3) βF-βG-M2e(23) and (A4) βH-βI-M2e(23). (b) SDS-PAGE analysis of M2e(7) and M2e(15) within βF-βG (B1,B3) and βH-βI(B2, B4) CCMV-CP loops. (c) SDS-PAGE (upper panel) and western blot (lower panel) of βF-βG M2e(7)/M2e(15) and βH-βI M2e(7)/M2e(15) detected by monoclonal anti-His-tag and M2e sera, respectively. (d) Immunoblot detection of VP1 and 2C fusions to CCMV-CP and NΔ24-CP using polyclonal anti-CCMV serum (D1) and serum from FMDV-infected guinea pig (D2). Induced empty pET-28a and CCMV-CP were used as negative and positive control, respectively. (e) Detection of M2e fused to NΔ24CP using polyclonal anti-CCMV (E1) and monoclonal anti-M2e (E2) sera. Molecular size marker is indicated on the left

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