Fig. 5
From: Feasibility of Cowpea chlorotic mottle virus-like particles as scaffold for epitope presentations
Schematic illustration of loop-inserted and coat protein fusion constructs expressed in E. coli. The ectodomain of M2e-protein of Influenza A virus: M2e(23), M2e(15) and M2e(7) introduced within the predicted loops (βB-βC-M2e(23), βD-βE-M2e(23), βF-βG-M2e(23)/M2e(15)/M2e(7) and βH-βI-(23)/M2e(15)/M2e(7) as adaptors via SpeI and SstI overhangs. Foot and mouth disease virus (VP1/2C) Influenza A virus epitopes [M2e(23) and HA] were fused by PCR to the N-terminus of the CCMV-CP (VP1/2C-CP) and NΔ24-CP (VP1/2C/M2e/HA-NΔ24-CP), and C-terminal to CCMV-CP (CP-HA, CP-M2e)