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Table 2 Isolation of IFN-CSP from E. coli inclusion bodies with a recovery protocol

From: High-level expression of a novel liver-targeting fusion interferon with preferred Escherichia coli codon preference and its anti-hepatitis B virus activity in vivo

Process step

Total protein

Purity

IFN-CSP

Recovery yield

(mg/L)

(%)

(mg/L)

(%)

Cell lysis

3512

61.62

2164.09

100.00

Centrifugation

2536

79.09

2005.72

92.68

Triton wash + Urea wash

1894

85.97

1628.27

81.18

Solubilization

1690

86.39

1459.99

89.60

Refolding

1359

87.16

1184.50

81.13

HiTrap affinity

735

99.85

733.89

61.96

Dialysis

691

99.85

690.00

93.73

  1. Total protein concentration was determined by Bradford method. Purity of IFN-CSP was calculated from densitometry analysis of SDS-PAGE gels. The concentration of IFN-CSP was calculated according the percentage fraction and total protein concentration