Process step | Total protein | Purity | IFN-CSP | Recovery yield |
---|---|---|---|---|
(mg/L) | (%) | (mg/L) | (%) | |
Cell lysis | 3512 | 61.62 | 2164.09 | 100.00 |
Centrifugation | 2536 | 79.09 | 2005.72 | 92.68 |
Triton wash + Urea wash | 1894 | 85.97 | 1628.27 | 81.18 |
Solubilization | 1690 | 86.39 | 1459.99 | 89.60 |
Refolding | 1359 | 87.16 | 1184.50 | 81.13 |
HiTrap affinity | 735 | 99.85 | 733.89 | 61.96 |
Dialysis | 691 | 99.85 | 690.00 | 93.73 |