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Table 2 Isolation of IFN-CSP from E. coli inclusion bodies with a recovery protocol

From: High-level expression of a novel liver-targeting fusion interferon with preferred Escherichia coli codon preference and its anti-hepatitis B virus activity in vivo

Process step Total protein Purity IFN-CSP Recovery yield
(mg/L) (%) (mg/L) (%)
Cell lysis 3512 61.62 2164.09 100.00
Centrifugation 2536 79.09 2005.72 92.68
Triton wash + Urea wash 1894 85.97 1628.27 81.18
Solubilization 1690 86.39 1459.99 89.60
Refolding 1359 87.16 1184.50 81.13
HiTrap affinity 735 99.85 733.89 61.96
Dialysis 691 99.85 690.00 93.73
  1. Total protein concentration was determined by Bradford method. Purity of IFN-CSP was calculated from densitometry analysis of SDS-PAGE gels. The concentration of IFN-CSP was calculated according the percentage fraction and total protein concentration