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Fig. 9 | BMC Biotechnology

Fig. 9

From: High-level expression of a novel liver-targeting fusion interferon with preferred Escherichia coli codon preference and its anti-hepatitis B virus activity in vivo

Fig. 9

Characterization of recombinant protein by SDS-PAGE, western blot, RP-HPLC and MALDI-MS. a: Purification of IFN-CSP. Lane M: Protein molecular weight marker, Lane 1–2: Total proteins of E. coli BL21/pET-21b-IFN-CSP before and after induction, Lane 3–4: Supernatant and precipitation after ultrasonication and centrifugation. Lane 5: Purified IFN-CSP using trion and urea wash. Lane 6: Purified IFN-CSP using HiTrap affinity chromatography. b: IFN-CSP was analyzed by western blot. Lane M: Protein molecular weight marker. Lane 1–2: Total proteins of E. coli BL21/pET-21b-IFN-CSP before and after induction. c: Analysis of purified IFN-CSP by RP-HPLC with a C18 column. d: Mass spectrum of purified IFN-CSP recorded on an Applied Biosytems Voyager MALDI-TOF mass spectrometry

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BMC Biotechnology

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