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Fig. 4 | BMC Biotechnology

Fig. 4

From: High-level expression of a novel liver-targeting fusion interferon with preferred Escherichia coli codon preference and its anti-hepatitis B virus activity in vivo

Fig. 4

Effect of temperature on the expression level of IFN-CSP in E. coli BL21/pET21b-IFN-CSP. a: SDS-PAGE analyses of IFN-CSP expression under different temperatures. Lane M: Protein molecular weight marker; Lane 1: E. coli BL21/pET21b-IFN-CSP before induction; Lanes 2–7: E. coli BL21/pET21b-IFN-CSP was cultured at 17, 22, 27, 32, 37 and 42 °C, respectively. b: Percentage and concentration of IFN-CSP were calculated by the target bands in SDS-PAGE (A)

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