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Fig. 2 | BMC Biotechnology

Fig. 2

From: In vivo tracking of human placenta derived mesenchymal stem cells in nude mice via 14C-TdR labeling

Fig. 2

Analysis of biological characteristics of PDB-MSCs after labeling. a The 4th passage of PDB-MSCs showed similar fibroblast-like morphology after cultivation in standard culture medium with and without 1 μCi 14C-TdR for 72h. Scale bar = 200μm. Differentiation capability of 1 μCi 14C-TdR labeled and unlabeled PDB-MSCs (b). Alizarin Red staining of differentiated (day 21) and control cells for osteogenic differentiation study, scale bar = 500μm. Oil red O staining of differentiated (day 8) and control cells for adipogenic differentiation study, scale bar =100μm. The CFU potential of 1 μCi 14C-TdR labeled and unlabeled PDB-MSCs at 8 days (c and d). Crystal violet (0.1 %) staining of 1 μCi 14C-TdR labeled (557 ± 20.1) and unlabeled (583 ± 32.5) PDB-MSCs shows no significant difference in colony-forming ability (n = 4) (P > 0.05). The 4th passage PDB-MSCs stained with PI were prepared for cell cycle analysis by NovoCyte flow cytometer and data were analyzed by NovoExpress analysis software. 1 μCi 14C-TdR labeled PDB-MSCs and unlabeled PDB-MSCs showed the similar trend and proportion of G0/G1, S and G2-M phase (e). Chromosome spread and karyotypic analysis of 1 μCi 14C-TdR labeled 4th passage PDB-MSCs (f). 22 pairs of somatic chromosomes were shown, as well as two X chromosomes, for a total of 46 chromosomes. Data were expressed as mean ± SD, N = 4 for CFU experiments

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