Fig. 4
From: Secretion of Fc-amidated peptide fusion proteins by Chinese hamster ovary cells
Characterization of Fc-fusion protein expression in CHO lines expressing PAM. Fc-AP-GLP1 (a) and Fc-GS-PYY (b) were expressed in PAM1 CHO cells; Fc-AP-NMU (c) was expressed in PAM820s CHO cells. Aliquots of cell extract (a, 5 %; b, 3 %; c, 6 % of total cell extract) and spent medium (a, 0.7 %; b, 1.3 %; c, 2.3 % of total medium volume) were prepared as described for Fig. 3. d Using GeneTools, data from several similar analyses were quantified to determine μg Fc/mg cell protein (d) and Fc secretion rate (e)