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Fig. 1 | BMC Biotechnology

Fig. 1

From: Secretion of Fc-amidated peptide fusion proteins by Chinese hamster ovary cells

Fig. 1

Design of vectors encoding Fc-peptidylglycine precursors. a Key features of the Fc-peptidylglycine precursors are illustrated. b The amino acid sequences of human GLP1-Gly, PYY-Gly and NMU-Gly are shown (http://www.bachem.com/research-products/). c Eadie-Hofstee plots illustrating the effects of synthetic PYY-Gly and GLP1-Gly on conversion of [125I]-Acetyl-Tyr-Val-Gly into [125I]-Acetyl-Try-Val-NH2 by purified PAM 820 s (0.085 ng/40 μl ≈ 1 fmol enzyme/tube). d Calculated kinetic parameters for PYY-Gly and GLP1-Gly. e pEAK Rapid cells were transiently transfected with vectors encoding Fc-GS-GLP1 (calculated mass discounting oligosaccharides; 31,720), Fc-AP-GLP1 (mass; 32,020), Fc-GS-PYY (mass; 32,730) and Fc-AP-PYY (mass; 33,090); control non-transfected cells (non) were analyzed at the same time. Aliquots of spent medium (20 μl) were fractionated by SDS-PAGE and visualized using antibody to human Fc; 18 ng human IgG (hIgG) was analyzed at the same time

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