Fig 6

Titration ELISA to determine reactivity of IgG from donor sera with I. scapularis metalloprotease (MP). MP was recombinantly produced in E. coli BLR(DE3), purified and immobilized in the wells of a microtiter plate. Reactivity of sera from donors 1, 2 and 8 is depicted (solid lines). As negative controls, sera were screened against blocked wells without recombinant MP (dashed lines)