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Fig 1 | BMC Biotechnology

Fig 1

From: Application of M13 phage display for identifying immunogenic proteins from tick (Ixodes scapularis) saliva

Fig 1

a Agarose gel electrophoresis of cDNA obtained after SMART cDNA synthesis using mRNA from salivary glands of 18 h fed I. scapularis nymphs. cDNA was subsequently used to construct the antigen library. b Colony PCR from randomly picked clones after cloning salivary gland derived cDNA fragments into pHORF3 and transformation of E. coli Top10F’ to determine insert percentage and size distribution of the antigen library

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BMC Biotechnology

ISSN: 1472-6750

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