Fig. 6

pyrG gene deletion in wild type and ∆ku70 mutant of M. thermophila ATCC 42464. The experiment design (a), PCR analysis of transformants under wild-type background (b) and ∆ku70 mutant (c) with one primer (pyrGout-F) located in the neo gene cassette and the other (pyrGout-R) located in the downstream of 3′ flank in the genomic DNA. The genomic DNA from the wild-type (WT) and ∆ku70 strains was used as the negative control