Fig. 2

Optimization of A. tumefaciens-mediated M. thermophila transformation. a The impact of acetosyringone (AS) on A. tumefaciens-mediated transformation efficiency. Co-cultivation was conducted in induction medium containing AS on indicated concentrations for 2 days. b The effect of co-culture time on transformation efficiency. Co-cultivation was conducted in induction medium containing 200 μM AS for various times indicated. Error bars indicate the standard deviation of three independent experiments