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Fig. 3 | BMC Biotechnology

Fig. 3

From: Rapid high-yield expression of a candidate influenza vaccine based on the ectodomain of M2 protein linked to flagellin in plants using viral vectors

Fig. 3

Expression and purification of Flg-4M protein. Coomassie brilliant blue stained gel (a) and western blot (b) of proteins isolated form N. benthamiana plants and separated by SDS-PAGE. M, − molecular weight marker (kD); 1, − total soluble proteins isolated from empty leaves; 2, − total soluble proteins isolated at fourth day after agroinfiltration from leaves infiltrated with vector pA7248AMV-Flg-4M; 3, − purified Flg-4M protein. Antibodies against M2e were used in western blotting

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