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Fig. 1 | BMC Biotechnology

Fig. 1

From: Rapid high-yield expression of a candidate influenza vaccine based on the ectodomain of M2 protein linked to flagellin in plants using viral vectors

Fig. 1

Structure of the expression constructs used in this study. a Amino acid sequence of Flg-4M protein. The sequences of human “consensus” M2e peptides, M2eh, are italicized; the sequences of M2e peptide of avian influenza virus strain A/Chicken/Kurgan/05/2005, M2ek, are shown in bold; the linker sequences are underlined. Flagellin sequence is shaded in grey. b Structures of viral vectors pA7248AMV-Flg-4M and pEAQ-Flg-4M. The tDNA regions of the binary vectors are shown. RDRP, RNA dependent RNA polymerase of PVX; Sgp1, subgenomic promoter of the PVX protein 25 K; AMV, the leader sequence of RNA 4 of the alfalfa mosaic virus; 6his, 6 histidine tag; FLG, flagellin of S. typhimurium; 4M2e, four copies of M2e peptide (arranged as M2eh-M2ek-M2eh-M2ek); 35S, promoter; 35S-T, terminator of the cauliflower mosaic virus RNA; NosT, terminator of nopaline synthase gene; LB and RB are the left and right borders of tDNA; p19, gene of silencing suppressor P19 from the tomato bushy stunt virus; 5’ and 3’, the 5’-UTR and the 3’-UTR from PVX (in pA7248AMV-Flg-4M) and CPMV (in pEAQ-Flg-4M)

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BMC Biotechnology

ISSN: 1472-6750

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