Fig. 4

SEC chromatograms of AVEBE MD20 and its products following incubation with GTFB preparations. Different GTFB preparations with equal hydrolysis activity (25.0 μg/ml soluble GTFB, 38.7 μg/ml refolded GTFB and 249.4 μg/ml 25 °C ncIB GTFB) were incubated with 5 % AVEBE MD20 for 72 h at 37 °C and pH 4.7. Elution volumes of the pullulan standards corresponding to 366, 200, 113, 48.8, 21.7, 10, 6.2, 1.32 and 0.342 kDa, are plotted above the x-axis